Paracetamol-Induced Splenic Toxicity and the Protective Role of Vitamin C in Albino Rats

Ahlaam M. Khalid; Eda M.A. Alshailabi; Ola A. Abdalally

doi:10.33214/pyyfqc31

Authors

Ahlaam M. Khalid Higher Institute of Medical Sciences and Technology, Laboratory Department, El-Beyda, Libya. Author
Eda M.A. Alshailabi Omar Al-Mukhtar University, Zoology Department, Faculty of Science, El-Beyda, Libya Author
Ola A. Abdalally Almahara institute for the medical and managerial sciences, El-Beyda, Libya. Author

DOI:

https://doi.org/10.33214/pyyfqc31

Keywords:

Paracetamol, Spleen, Vitamin C, Albino rats, Histopathology.

Abstract

Paracetamol (PCM) is widely used as an analgesic and antipyretic agent; however, overdose can result in systemic toxicity, primarily mediated through oxidative stress. This study aimed to evaluate the histopathological effects of PCM on the spleen of albino rats and to assess the potential protective effect of vitamin C (VC) as an antioxidant. Twenty-eight albino rats were randomly assigned to five groups: control, VC (500 mg/kg), PCM (500 mg/kg), PCM + VC co-treatment, and a prophylactic group receiving VC before PCM administration. Histological examination of the spleen revealed alterations in the PCM-only group, including lymphoid depletion in the white pulp and congestion in the red pulp. Co-administration of VC attenuated these changes, while pre-treatment with VC provided the greatest preservation of splenic architecture. These findings suggest a potential protective benefit of VC against PCM-induced oxidative damage in the spleen

REFERENCES

Alshailabi, E. M. A. (2016). Effects of omeprazole on healing of non-steroidal anti-inflammatory drug (NSAID)-induced peptic ulceration in rats and protective role of indole-3-carbinol. Journal of Sciences and Humanities Studies, 12, 174–188.

Alshailabi, E. M. A., Abdalally, O. A., & Majeed, S. F. (2021). Histopathological study on the protective effect of vitamin C against paracetamol-induced acute hepatic damage in rat. Global Libyan Journal, 53, 1–15.

Alshailabi, E. M., Abdalally, O. A., & Mohammed, F. A. (2024). The protective role of ascorbic acid on the testis tissue damage induced by paracetamol in albino rats. Al-Kitab Journal for Pure Sciences, 8(1), 19–28. https://doi.org/10.32441/kjps.08.01.p3

Bancroft, J. D., & Gamble, M. (2008). Theory and practice of histological techniques (6th ed.). Churchill Livingstone, Elsevier.

Başeğmez, M., Eryavuz, A., & Demirel, H. H. (2025). Effects of vitamin C supplementation on total antioxidant status, inflammation, and histopathological changes in aged rats. Journal of Biochemical and Molecular Toxicology, 39(6), e70324. https://doi.org/10.1002/jbt.70324

Du, K., Ramachandran, A., & Jaeschke, H. (2016). Oxidative stress during acetaminophen hepatotoxicity: Sources, pathophysiological role and therapeutic potential. Redox Biology, 10, 148–156. https://doi.org/10.1016/j.redox.2016.10.001

Elduob, R. E. A., Alshailabi, E. M. A., & Efkeren, S. M. (2023). The protective effects of rutin and stem cells against the kidney function changes induced by paracetamol in rats. Sirte University Scientific Journal, 13, 53–58. https://doi.org/10.37375/susj.v13i2.2501

Elmore, S. A. (2006). Enhanced histopathology of the spleen. Toxicologic Pathology, 34(5). https://doi.org/10.1080/0192623060086552

Jaeschke, H., Duan, L., Akakpo, J. Y., Farhood, A., & Ramachandran, A. (2018). The role of apoptosis in acetaminophen hepatotoxicity. Food and Chemical Toxicology, 118, 709–718. https://doi.org/10.1016/j.fct.2018.06.025

Kennon-McGill, S., & McGill, M. R. (2017). Extrahepatic toxicity of acetaminophen: Critical evaluation of the evidence and proposed mechanisms. Journal of Clinical and Translational Research, 3(3), 297–310. https://doi.org/10.18053/jctres.03.201703.005

Kozlov, A. V., Javadov, S., & Sommer, N. (2024). Cellular ROS and antioxidants: Physiological and pathological role. Antioxidants, 13(5), 602. https://doi.org/10.3390/antiox13050602

Landmann, M., Scheibner, D., Graaf, A., Gischke, M., Koethe, S., Fatola, O. I., Raddatz, B., Mettenleiter, T. C., Beer, M., Grund, C., Harder, T., Abdelwhab, E. M., & Ulrich, R. (2021). A semiquantitative scoring system for histopathological and immunohistochemical assessment of lesions and tissue tropism in avian influenza. Viruses, 13(5), 868. https://doi.org/10.3390/v13050868

Lee, E., Park, H.-Y., Kim, S.-W., Kim, J., & Lim, K. (2023). Vitamin C and glutathione supplementation: A review of their additive effects on exercise performance. Physical Activity and Nutrition, 27(3), 36–43. https://doi.org/10.20463/pan.2023.0027

Mandal, M., Gardner, C. R., Sun, R., Choi, H., Lad, S., Mishin, V., Laskin, J. D., & Laskin, D. L. (2016). The spleen as an extramedullary source of inflammatory cells responding to acetaminophen-induced liver injury. Toxicology and Applied Pharmacology, 304, 110–120. https://doi.org/10.1016/j.taap.2016.04.019

Manful, C. F., Fordjour, E., Ikumoinein, E., Abbey, L., & Thomas, R. (2025). Therapeutic strategies targeting oxidative stress and inflammation: A narrative review. BioChem, 5(4), 35. https://doi.org/10.3390/biochem5040035

Ogunleye, O. D., Afolabi, O. A., Saka, W. A., Olusola, B. O., Ajike, R. A., Oladokun, O. O., Hammed, S. O., Hezekiah, O. S., & Adedeji, O. J. (2025). Possible role of vitamins C and E co-administration in the prevention of testicular ischemia–reperfusion injury following surgical repair of torsion of the testis. Frontiers in Nutrition, 12. https://doi.org/10.3389/fnut.2025.1660240

Rössig, L., Hoffmann, J., Hügel, B., Mallat, Z., Haase, A., Freyssinet, J. M., Tedgui, A., Aicher, A., Zeiher, A. M., & Dimmeler, S. (2001). Vitamin C inhibits endothelial cell apoptosis in congestive heart failure. Circulation, 104(18), 2182–2187. https://doi.org/10.1161/hc4301.098284

Venkatesan, P. S., Deecaraman, M., Vijayalakshmi, M., & Sakthivelan, S. M. (2014). Sub-acute toxicity studies of acetaminophen in Sprague Dawley rats. Biological & Pharmaceutical Bulletin, 37(7), 1184–1190. https://doi.org/10.1248/bpb.b14-00066